Fitness of in vitro selected Pseudomonas aeruginosa nalB and nfxB multidrug resistant mutants. Academic Article uri icon

Overview

abstract

  • Overproduction of multidrug resistance (MDR) efflux pumps is involved in the resistance to a wide range of compounds in bacteria. These determinants extrude antibiotics, but also bacterial metabolites like quorum-sensing signals. Non-regulated extrusion of bacterial metabolites might produce a metabolic burden, so that MDR-overproducing mutants could have a reduced fitness when compared with their parental strains. To test such a possibility, we have compared the behaviour of two MDR Pseudomonas aeruginosa in vitro selected mutants (nalB and nfxB) with their isogenic parental strain with respect to some properties with potential relevance for the survival in the environment and the virulence of this bacterial species. Overproduction of the MDR determinants MexABOprM (nalB mutant) and MexCDOprJ (nfxB mutant) decreased the survival in water, the production of phenazines and proteases, and the virulence (using a Caenorhabditis elegans model system) of the P. aeruginosa mutants. In contrast, the capability of forming biofilms was not impaired. The simple models tested in the present work can enable the analysis of the fitness of large numbers of antibiotic-resistant bacteria by using more realistic approaches than the in vitro competition assays currently used.

publication date

  • November 1, 2002

Research

keywords

  • Drug Resistance, Multiple, Bacterial
  • Mutation
  • Pseudomonas aeruginosa

Identity

Scopus Document Identifier

  • 0036849364

PubMed ID

  • 12407121

Additional Document Info

volume

  • 50

issue

  • 5