Preliminary characterization of Pseudomonas aeruginosa peptide chemotactins for polymorphonuclear leukocytes. Academic Article uri icon

Overview

abstract

  • In a previous report, we showed that supernatants of Pseudomonas aeruginosa cultures exhibit chemotactic activity for polymorphonuclear leukocytes (PMNL). In this study, P. aeruginosa chemotactins were isolated, purified, and partially characterized. The organisms were cultured in Vogel-Bonner defined medium, and cultures were stopped in late log phase. Chemotactins withstood heating, remained unaltered after acid or alkali treatment in a pH range from 4 to 10, and resisted digestion by trypsin or carboxypeptidase, but chemotactic activity was decreased by 73% after incubation with pronase. Only 2% of the total chemotactic activity of culture supernatants could be extracted with chloroform. Chemotactins with molecular sizes less than 3 kDa constituted the largest contribution to the chemotactic activity of culture supernatants. Pretreatment of PMNL with 10(-5) M formylmethionyl-leucyl-phenylalanine (FMLP) inhibited chemotaxis towards FMLP and P. aeruginosa culture supernatants but not towards complement component C5a. In conclusion, the total chemotactic activity for PMNL of P. aeruginosa culture supernatants was due, almost exclusively, to chemotactins that have properties similar, if not identical, to those exhibited by formylmethionyl peptides.

publication date

  • June 1, 1992

Research

keywords

  • Bacterial Proteins
  • Chemotactic Factors
  • Chemotaxis, Leukocyte
  • Neutrophils
  • Pseudomonas aeruginosa

Identity

PubMed Central ID

  • PMC257182

Scopus Document Identifier

  • 0026753082

Digital Object Identifier (DOI)

  • 10.1128/iai.60.6.2465-2469.1992

PubMed ID

  • 1587615

Additional Document Info

volume

  • 60

issue

  • 6