Modified bacterial artificial chromosomes for zebrafish transgenesis. Academic Article uri icon

Overview

abstract

  • Transgenesis using bacterial artificial chromosomes (BAC) offers greater fidelity in directing desirable expression of foreign genes. Application of this technology in the optically transparent zebrafish with fluorescent protein reporters enables unparalleled visual analysis of regulation of gene expression in a living organism. Here we describe a streamlined procedure of direct selecting multiple BAC clones based on public sequence databases followed by rapid modification with GFP or RFP for transgenic analysis in zebrafish. Experimental procedures for BAC DNA preparation, microinjection of zebrafish embryos and screening of transgenic zebrafish carrying GFP/RFP modified BAC clones are detailed.

publication date

  • July 1, 2006

Research

keywords

  • Animals, Genetically Modified
  • Chromosomes, Artificial, Bacterial
  • Genetic Engineering
  • Zebrafish

Identity

Scopus Document Identifier

  • 33746875204

PubMed ID

  • 16828309

Additional Document Info

volume

  • 39

issue

  • 3