Deep tissue multiphoton microscopy using longer wavelength excitation. Academic Article uri icon

Overview

abstract

  • We compare the maximal two-photon fluorescence microscopy (TPM) imaging depth achieved with 775-nm excitation to that achieved with 1280-nm excitation through in vivo and ex vivo TPM of fluorescently-labeled blood vessels in mouse brain. We achieved high contrast imaging of blood vessels at approximately twice the depth with 1280-nm excitation as with 775-nm excitation. An imaging depth of 1 mm can be achieved in in vivo imaging of adult mouse brains at 1280 nm with approximately 1-nJ pulse energy at the sample surface. Blood flow speed measurements at a depth of 900 mum are performed.

publication date

  • August 3, 2009

Research

keywords

  • Image Enhancement
  • Lighting
  • Microscopy, Fluorescence, Multiphoton

Identity

Scopus Document Identifier

  • 68349144553

PubMed ID

  • 19654740

Additional Document Info

volume

  • 17

issue

  • 16