Imaging single molecular motor motility with total internal reflection fluorescence microscopy (TIRFM). Academic Article uri icon

Overview

abstract

  • Total internal reflection fluorescence microscopy (TIRFM) allows fluorescent molecules on or near the plasma membrane to be visualized with a very high signal-to-noise ratio. This strategy has been very successful for imaging molecular machines as they move and do work. We provide here a general protocol for imaging single molecular motors as they move along microtubule tracks. Our protocol is designed for the study of cytoplasmic dynein purified from Saccharomyces cerevisiae, but it represents a general framework for any in vitro single-molecule assay.

publication date

  • March 1, 2010

Research

keywords

  • Cell Membrane
  • Cytoplasm
  • Image Processing, Computer-Assisted
  • Microscopy, Fluorescence
  • Molecular Motor Proteins

Identity

Digital Object Identifier (DOI)

  • 10.1101/pdb.prot5399

PubMed ID

  • 20194468

Additional Document Info

volume

  • 2010

issue

  • 3