The effects of DNA methyltransferase inhibitors and histone deacetylase inhibitors on digit regeneration in mice. Academic Article uri icon

Overview

abstract

  • METHOD: We injected two drugs that modify the epigenome, the DNA methyltransferase inhibitor 5-aza-2'-deoxycytidine (5-aza-dC) and the histone deacetylase inhibitor trichostatin A (TSA), alone or in combination, into C57Bl/6 mice subjected to amputation through the mid-second phalanx of the third digit. Wound-site tissue was collected. RESULTS: We observed increased staining of the stem cell markers Rex1 (Zfp42) and stem cell antigen-1 at digit amputation sites from drug-treated mice. Samples from 5-aza-dC plus TSA and TSA treated mice also showed increased proliferating cell nuclear antigen staining, a measure of cell proliferation. Drug treatments increased Msx1, but not Cyp26a1 or ALDH1a2 (RALDH2) mRNA. CONCLUSION: 5-aza-dC and TSA treatments stimulated cell proliferation at the amputation site, possibly via increased expression of genes involved in digit development and regeneration.

publication date

  • March 1, 2010

Research

keywords

  • Histone Deacetylase Inhibitors
  • Methyltransferases
  • Regeneration
  • Toes

Identity

Scopus Document Identifier

  • 77949368108

Digital Object Identifier (DOI)

  • 10.2217/rme.09.91

PubMed ID

  • 20210581

Additional Document Info

volume

  • 5

issue

  • 2