The accumulation of H-1 MR-visible lipid in human glioma cells is independent of the cell cycle.

Overview

The human glioma cell line, 2607, was observed with proton magnetic resonance spectroscopy (MRS) in the presence of a number of cell-cycle blockers (cysteamine, desferrioxamine, ADR 529). MR spectra of the arrested cells revealed a correlation between the intensity of the lipid methylene resonance at 1.3 ppm and the percentage of cells in G(2)/M. However, subsequent time course studies using ADR 529 on cells partially synchronised by contact inhibition showed that the emergence of the lipid signal in drug-treated cells is not strictly cell cycle dependent but increases with continuing exposure to ADR 529. This indicates that the accumulation of MR-visible lipid arises from drug cell interactions that specifically affect lipid metabolism in a non cell cycle dependent manner.