Cargo ubiquitination is essential for multivesicular body intralumenal vesicle formation. Academic Article uri icon

Overview

abstract

  • The efficient formation of a variety of transport vesicles is influenced by the presence of cargo, suggesting that cargo itself might have a defining role in vesicle biogenesis. However, definitive in vivo experiments supporting this concept are lacking, as it is difficult to eliminate endogenous cargo. The Endosomal Sorting Complexes Required for Transport (ESCRT) apparatus sorts ubiquitinated membrane proteins into endosomal intralumenal vesicles (ILVs) that accumulate within multivesicular bodies. Here we show that cargo ubiquitination is required for effective recruitment of the ESCRT machinery onto endosomal membranes and for the subsequent formation of ILVs.

publication date

  • April 1, 2012

Research

keywords

  • Multivesicular Bodies
  • Saccharomyces cerevisiae
  • Ubiquitination

Identity

PubMed Central ID

  • PMC3321151

Scopus Document Identifier

  • 84859430548

Digital Object Identifier (DOI)

  • 10.1038/embor.2012.18

PubMed ID

  • 22370727

Additional Document Info

volume

  • 13

issue

  • 4