Unzipping single DNA molecules to study nucleosome structure and dynamics. Article uri icon

Overview

abstract

  • DNA unzipping is a powerful tool to study protein-DNA interactions at the single-molecule level. In this chapter, we provide a detailed and practical guide to performing this technique with an optical trap, using nucleosome studies as an example. We detail protocols for preparing an unzipping template, constructing and calibrating the instrument, and acquiring, processing, and analyzing unzipping data. We also summarize major results from utilization of this technique for the studies of nucleosome structure, dynamics, positioning, and remodeling.

publication date

  • January 1, 2012

Research

keywords

  • DNA
  • Nucleosomes
  • Optical Tweezers

Identity

PubMed Central ID

  • PMC5515249

Scopus Document Identifier

  • 84865472310

Digital Object Identifier (DOI)

  • 10.1016/B978-0-12-391938-0.00002-1

PubMed ID

  • 22929764

Additional Document Info

volume

  • 513