Application of an efficient gene targeting system linking secondary metabolites to their biosynthetic genes in Aspergillus terreus. Academic Article uri icon

Overview

abstract

  • Nonribosomal peptides (NRPs) are natural products biosynthesized by NRP synthetases. A kusA-, pyrG- mutant strain of Aspergillus terreus NIH 2624 was developed that greatly facilitated the gene targeting efficiency in this organism. Application of this tool allowed us to link four major types of NRP-related secondary metabolites to their responsible genes in A. terreus. In addition, an NRP affecting melanin synthesis was also identified in this species.

publication date

  • July 10, 2013

Research

keywords

  • Aspergillus
  • Peptide Biosynthesis, Nucleic Acid-Independent
  • Peptide Synthases

Identity

PubMed Central ID

  • PMC3833251

Scopus Document Identifier

  • 84880566602

Digital Object Identifier (DOI)

  • 10.1021/ol401384v

PubMed ID

  • 23841722

Additional Document Info

volume

  • 15

issue

  • 14