Essential N-terminal insertion motif anchors the ESCRT-III filament during MVB vesicle formation. Academic Article uri icon

Overview

abstract

  • The endosomal sorting complexes required for transport (ESCRTs) have emerged as key cellular machinery that drive topologically unique membrane deformation and scission. Understanding how the ESCRT-III polymer interacts with membrane, promoting and/or stabilizing membrane deformation, is an important step in elucidating this sculpting mechanism. Using a combination of genetic and biochemical approaches, both in vivo and in vitro, we identify two essential modules required for ESCRT-III-membrane association: an electrostatic cluster and an N-terminal insertion motif. Mutating either module in yeast causes cargo sorting defects in the MVB pathway. We show that the essential N-terminal insertion motif provides a stable anchor for the ESCRT-III polymer. By replacing this N-terminal motif with well-characterized membrane insertion modules, we demonstrate that the N terminus of Snf7 has been tuned to maintain the topological constraints associated with ESCRT-III-filament-mediated membrane invagination and vesicle formation. Our results provide insights into the spatially unique, ESCRT-III-mediated membrane remodeling.

publication date

  • October 17, 2013

Research

keywords

  • Cell Membrane
  • Endosomal Sorting Complexes Required for Transport
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins

Identity

Scopus Document Identifier

  • 84886641339

Digital Object Identifier (DOI)

  • 10.1016/j.devcel.2013.09.009

PubMed ID

  • 24139821

Additional Document Info

volume

  • 27

issue

  • 2