A modular labeling strategy for in vivo PET and near-infrared fluorescence imaging of nanoparticle tumor targeting. Academic Article uri icon

Overview

abstract

  • UNLABELLED: Advances in preclinical molecular imaging have generated new opportunities to noninvasively visualize the biodistribution and tumor targeting of nanoparticle therapeutics. Capitalizing on recent achievements in this area, we sought to develop an (89)Zr-based labeling strategy for liposomal nanoparticles that accumulate in tumors via passive targeting mechanisms. METHODS: (89)Zr-labeled liposomes were prepared using 2 different approaches: click labeling and surface chelation. Pharmacokinetic and biodistribution studies, as well as PET/CT imaging of the radiolabeled nanoparticles, were performed on a mouse model of breast cancer. In addition, a dual PET/optical probe was prepared by incorporation of a near-infrared fluorophore and tested in vivo by PET and near-infrared fluorescence imaging. RESULTS: The surface chelation approach proved to be superior in terms of radiochemical yield and stability, as well as in vivo performance. Accumulation of these liposomes in tumor peaked at 24 h after injection and was measured to be 13.7 ± 1.8 percentage injected dose per gram. The in vivo performance of this probe was not essentially perturbed by the incorporation of a near-infrared fluorophore. CONCLUSION: We have developed a highly modular and efficient strategy for the labeling of liposomal nanoparticles with (89)Zr. In xenograft and orthotopic mouse models of breast cancer, we demonstrated that the biodistribution of these nanoparticles can be visualized by PET imaging. In combination with a near-infrared dye, these liposomal nanoparticles can serve as bimodal PET/optical imaging agents. The liposomes target malignant growth, and their bimodal features may be useful for simultaneous PET and intraoperative imaging.

publication date

  • July 24, 2014

Research

keywords

  • Fluorescent Dyes
  • Nanoparticles
  • Neoplasms
  • Positron-Emission Tomography
  • Zirconium

Identity

PubMed Central ID

  • PMC4381653

Scopus Document Identifier

  • 84907558689

Digital Object Identifier (DOI)

  • 10.2967/jnumed.114.141861

PubMed ID

  • 25060196

Additional Document Info

volume

  • 55

issue

  • 10