An in-house assay is superior to Sepsityper for direct matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry identification of yeast species in blood cultures. Academic Article uri icon

Overview

abstract

  • We developed an in-house assay for the direct identification, by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry, of yeasts in blood culture. Sixty-one representative strains from 12 species were analyzed in spiked blood cultures. Our assay accurately identified 95 of 107 (88.8%) positive blood cultures and outperformed the commercial Sepsityper kit (81.7% identification).

publication date

  • March 11, 2015

Research

keywords

  • Blood
  • Fungemia
  • Microbiological Techniques
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Yeasts

Identity

PubMed Central ID

  • PMC4400771

Scopus Document Identifier

  • 84928014420

Digital Object Identifier (DOI)

  • 10.1128/JCM.03600-14

PubMed ID

  • 25762771

Additional Document Info

volume

  • 53

issue

  • 5