Temporal stability and molecular persistence of the bone marrow plasma cell antibody repertoire. Academic Article uri icon

Overview

abstract

  • Plasma cells in human bone marrow (BM) are thought to be responsible for sustaining lifelong immunity, but its underlying basis is controversial. Here we use high-throughput sequence analysis of the same individual across 6.5 years to show that the BM plasma cell immunoglobulin heavy chain repertoire is remarkably stable over time. We find a nearly static bias in individual and combinatorial gene usage across time. Analysis of a second donor corroborates these observations. We also report the persistence of numerous BM plasma cell clonotypes (∼2%) identifiable at all points assayed across 6.5 years, supporting a model of serological memory based upon intrinsic longevity of human plasma cells. Donors were adolescents who completely recovered from neuroblastoma prior to the start of this study. Our work will facilitate differentiation between healthy and diseased antibody repertoires, by serving as a point of comparison with future deep-sequencing studies involving immune intervention.

publication date

  • December 21, 2016

Research

keywords

  • Antibody Formation
  • Bone Marrow
  • Bone Marrow Cells
  • Plasma Cells

Identity

PubMed Central ID

  • PMC5187582

Scopus Document Identifier

  • 85006932864

Digital Object Identifier (DOI)

  • 10.1038/ncomms13838

PubMed ID

  • 28000661

Additional Document Info

volume

  • 7