In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain. Academic Article uri icon

Overview

abstract

  • High-resolution optical imaging is critical to understanding brain function. We demonstrate that three-photon microscopy at 1,300-nm excitation enables functional imaging of GCaMP6s-labeled neurons beyond the depth limit of two-photon microscopy. We record spontaneous activity from up to 150 neurons in the hippocampal stratum pyramidale at ∼1-mm depth within an intact mouse brain. Our method creates opportunities for noninvasive recording of neuronal activity with high spatial and temporal resolution deep within scattering brain tissues.

publication date

  • February 20, 2017

Research

keywords

  • Brain
  • Microscopy, Fluorescence, Multiphoton
  • Neurons

Identity

PubMed Central ID

  • PMC6441362

Scopus Document Identifier

  • 85013224083

Digital Object Identifier (DOI)

  • 10.1038/nmeth.4183

PubMed ID

  • 28218900

Additional Document Info

volume

  • 14

issue

  • 4