Multiplex Reverse Transcription-PCR for Simultaneous Surveillance of Influenza A and B Viruses. Academic Article uri icon

Overview

abstract

  • Influenza A and B viruses are the causative agents of annual influenza epidemics that can be severe, and influenza A viruses intermittently cause pandemics. Sequence information from influenza virus genomes is instrumental in determining mechanisms underpinning antigenic evolution and antiviral resistance. However, due to sequence diversity and the dynamics of influenza virus evolution, rapid and high-throughput sequencing of influenza viruses remains a challenge. We developed a single-reaction influenza A/B virus (FluA/B) multiplex reverse transcription-PCR (RT-PCR) method that amplifies the most critical genomic segments (hemagglutinin [HA], neuraminidase [NA], and matrix [M]) of seasonal influenza A and B viruses for next-generation sequencing, regardless of viral type, subtype, or lineage. Herein, we demonstrate that the strategy is highly sensitive and robust. The strategy was validated on thousands of seasonal influenza A and B virus-positive specimens using multiple next-generation sequencing platforms.

publication date

  • October 4, 2017

Research

keywords

  • Influenza A virus
  • Influenza B virus
  • Influenza, Human
  • Multiplex Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction

Identity

PubMed Central ID

  • PMC5703814

Scopus Document Identifier

  • 85036621703

Digital Object Identifier (DOI)

  • 10.1128/JCM.00957-17

PubMed ID

  • 28978683

Additional Document Info

volume

  • 55

issue

  • 12