Interferon alpha associated with systemic lupus erythematosus is not intrinsically acid labile. Academic Article uri icon

Overview

abstract

  • The physicochemical properties of apparently acid-labile IFN-alpha from patients with SLE have been studied. The antigenicity, apparent molecular size, and isoelectric point of SLE IFN-alpha are indistinguishable from those of conventional, previously characterized, acid-stable subspecies of IFN-alpha. However, after partial purification by anion-exchange chromatography, SLE IFN-alpha no longer exhibits acid lability, suggesting that other plasma factor(s) are responsible for the acid lability of SLE IFN-alpha. Addition of SLE plasma, but not normal plasma, to conventional acid-stable IFN-alpha renders the exogenous IFN-alpha acid labile. Preliminary results demonstrate that an acid-dependent IFN-inactivating activity can be partially purified from SLE plasma by anion-exchange chromatography.

publication date

  • March 1, 1989

Research

keywords

  • Interferon Type I
  • Lupus Erythematosus, Systemic

Identity

PubMed Central ID

  • PMC2189267

Scopus Document Identifier

  • 0024504970

PubMed ID

  • 2926326

Additional Document Info

volume

  • 169

issue

  • 3