Determinants of Tenascin-C and HIV-1 envelope binding and neutralization. Academic Article uri icon

Overview

abstract

  • Interactions between innate antiviral factors at mucosal surfaces and HIV-1 virions contribute to the natural inefficiency of HIV-1 transmission and are a platform to inform the development of vaccine and nonvaccine strategies to block mucosal HIV-1 transmission. Tenascin-C (TNC) is a large, hexameric extracellular matrix glycoprotein identified in breast milk and genital fluids that broadly neutralizes HIV-1 via interaction with the HIV-1 Envelope (Env) variable 3 (V3) loop. In this report, we characterize the specific determinants of the interaction between TNC and the HIV-1 Env. We observed that TNC binding and neutralization of HIV-1 is dependent on the TNC fibrinogen-like globe (fbg) and fibronectin-type III (fn) domains, oligomerization, and its newly-mapped glycan structure. Moreover, we observed that TNC-mediated neutralization is also dependent on Env V3 residues 321/322 and 326/327, which surround the IGDIR motif of the V3 loop, as well the N332 glycan, which is critical to the broadly neutralizing activity of glycan-dependent V3-specific antibodies such as PGT128. Our results demonstrate a striking parallel between innate and adaptive immune mechanisms of broad HIV neutralization and provide further insight into the host protein-virus interactions responsible for the natural inefficiency of mucosal HIV-1 transmission.

publication date

  • April 11, 2019

Research

keywords

  • HIV-1
  • Tenascin
  • env Gene Products, Human Immunodeficiency Virus

Identity

PubMed Central ID

  • PMC6599478

Scopus Document Identifier

  • 85064230451

Digital Object Identifier (DOI)

  • 10.1038/s41385-019-0164-2

PubMed ID

  • 30976088

Additional Document Info

volume

  • 12

issue

  • 4