Two-Step Bacterial Artificial Chromosome (BAC) Engineering: Preparation of Shuttle Vector DNA. Academic Article uri icon

Overview

abstract

  • In two-step bacterial artificial chromosome (BAC) engineering, a single plasmid is introduced into the BAC-carrying cell lines. The shuttle vector pLD53.SCAB (or pLD53.SCAEB) carries the recA gene and the R6Kγ origin, which requires the π protein to replicate. PIR2 cells, expressing π, are typically used for the amplification of the vector and maintain about 15 copies/cell of the donor vector, which is relatively stable in this host.

publication date

  • April 1, 2020

Research

keywords

  • Chromosomes, Artificial, Bacterial
  • Genetic Engineering
  • Genetic Vectors
  • Plasmids

Identity

Scopus Document Identifier

  • 85082979245

Digital Object Identifier (DOI)

  • 10.1101/pdb.prot098038

PubMed ID

  • 32238589

Additional Document Info

volume

  • 2020

issue

  • 4