One-Step Bacterial Artificial Chromosome (BAC) Modification: Transformation of the BAC Host with the RecA Vector. Academic Article uri icon

Overview

abstract

  • This protocol outlines the steps for introducing the RecA plasmid into bacterial artificial chromosome (BAC) host cells, and their preparation for subsequent transformation with the reporter plasmid for one-step BAC modification. BAC host cells are rendered chemically competent and transformed with the RecA plasmid, and transformants are selected for tetracycline resistance to ensure the presence of the RecA marker.

publication date

  • July 1, 2020

Research

keywords

  • Chromosomes, Artificial, Bacterial
  • Escherichia coli
  • Genetic Vectors
  • Plasmids
  • Rec A Recombinases
  • Transformation, Bacterial

Identity

Scopus Document Identifier

  • 85087474270

Digital Object Identifier (DOI)

  • 10.1101/pdb.prot098129

PubMed ID

  • 32611779

Additional Document Info

volume

  • 2020

issue

  • 7