Fungal Bioreporters to Monitor Outcomes of Aspergillus: Host-Cell Interactions. uri icon

Overview

abstract

  • Fluorescence-based techniques enable researchers to monitor physiologic processes, specifically fungal cell viability and death, during cellular encounters with the mammalian immune system with single event resolution. By incorporating two independent fluorescent probes in fungal organisms either prior to, or ensuing experimental infection in mice or in cultured leukocytes, it is possible to distinguish and quantify live and killed fungal cells to interrogate genetic, pharmacologic, and cellular determinants that shape host-fungal cell outcomes. This chapter reviews the techniques and applications of fluorescent fungal reporters of viability, with emphasis on the filamentous mold Aspergillus fumigatus.

publication date

  • January 1, 2021

Research

keywords

  • Aspergillus fumigatus
  • Gene Expression Regulation, Fungal
  • Genes, Reporter
  • Leukocytes
  • Luminescent Proteins
  • Lung
  • Microscopy, Fluorescence

Identity

PubMed Central ID

  • PMC9088164

Scopus Document Identifier

  • 85100125095

Digital Object Identifier (DOI)

  • 10.1007/978-1-0716-1182-1_8

PubMed ID

  • 33405034

Additional Document Info

volume

  • 2260