A protocol for qualitative and quantitative measurement of endosomal processing using hot spot analysis. Academic Article uri icon

Overview

abstract

  • A detailed quantification of antigen processing by endosomal compartments provides important information on the pattern of protein fragmentation. Here, we describe a protocol that combines gradient purified endosomes, incubated with antigens, followed by hot spot analysis of MS/MS-sequenced peptides. The analysis identifies differences in endosomal antigen processing by dendritic cells under diverse experimental conditions. For complete details on the use and execution of this protocol, please refer to Clement et al. (2021).

publication date

  • July 6, 2021

Research

keywords

  • Antigens
  • Endosomes
  • Molecular Biology

Identity

PubMed Central ID

  • PMC8264744

Scopus Document Identifier

  • 85110113675

Digital Object Identifier (DOI)

  • 10.1016/j.xpro.2021.100648

PubMed ID

  • 34278334

Additional Document Info

volume

  • 2

issue

  • 3