Redundant cytokine requirement for intestinal microbiota-induced Th17 cell differentiation in draining lymph nodes. Academic Article uri icon

Overview

abstract

  • Differentiation of intestinal T helper 17 (Th17) cells, which contribute to mucosal barrier protection from invasive pathogens, is dependent on colonization with distinct commensal bacteria. Segmented filamentous bacteria (SFB) are sufficient to support Th17 cell differentiation in mouse, but the molecular and cellular requirements for this process remain incompletely characterized. Here, we show that intestine-draining mesenteric lymph nodes (MLNs), not intestine proper, are the dominant site of SFB-induced intestinal Th17 cell differentiation. Subsequent migration of these cells to the intestinal lamina propria is dependent on their upregulation of integrin β7. Stat3-dependent induction of RORγt, the Th17 cell-specifying transcription factor, largely depends on IL-6, but signaling through the receptors for IL-21 and IL-23 can compensate for absence of IL-6 to promote SFB-directed Th17 cell differentiation. These results indicate that redundant cytokine signals guide commensal microbe-dependent Th17 cell differentiation in the MLNs and accumulation of the cells in the lamina propria.

publication date

  • August 24, 2021

Research

keywords

  • Cell Differentiation
  • Cytokines
  • Intestines
  • Lymph Nodes
  • Th17 Cells

Identity

PubMed Central ID

  • PMC8845566

Scopus Document Identifier

  • 85113412466

Digital Object Identifier (DOI)

  • 10.1016/j.celrep.2021.109608

PubMed ID

  • 34433045

Additional Document Info

volume

  • 36

issue

  • 8