Stem cell-derived cardiomyocyte heterogeneity confounds electrophysiological insights. Review uri icon

Overview

abstract

  • Human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) offer potential as an in vitro model for studying drug cardiotoxicity and patient-specific cardiovascular disease. The inherent electrophysiological heterogeneity of these cells limits the depth of insights that can be drawn from well-designed experiments. In this review, we provide our perspective on some sources and the consequences of iPSC-CM heterogeneity. We demonstrate the extent of heterogeneity in the literature and explain how such heterogeneity is exacerbated by patch-clamp experimental artifacts in the manual and automated set-up. Finally, we discuss how this heterogeneity, caused by both intrinsic and extrinsic factors, limits our ability to build digital twins of patient-derived cardiomyocytes.

publication date

  • May 9, 2024

Research

keywords

  • Induced Pluripotent Stem Cells
  • Myocytes, Cardiac

Identity

PubMed Central ID

  • PMC11493526

Scopus Document Identifier

  • 85192525880

Digital Object Identifier (DOI)

  • 10.1113/JP284618

PubMed ID

  • 38723234

Additional Document Info

volume

  • 602

issue

  • 20