UDP-N-acetylglucosamine 1-carboxyvinyl-transferase from Acinetobacter calcoaceticus. Academic Article uri icon

Overview

abstract

  • We have analyzed the sequence downstream of rpoN from Acinetobacter calcoaceticus and identified an open reading frame encoding a protein with high similarity to UDP-N-acetylglucosamine 1-carboxyvinyl-transferase (MurZ). Multicopy plasmids encoding this enzyme conferred phosphomycin resistance to A. calcoaceticus. The polar effect of a rpoN mutation on the phosphomycin resistance level suggests that murZ is, in part, cotranscribed with rpoN. These observations confirm that A. calcoaceticus represents the first exception from a conserved genetic context of rpoN observed in several other Gram-negative bacteria.

publication date

  • April 1, 1994

Research

keywords

  • Acinetobacter calcoaceticus
  • Alkyl and Aryl Transferases
  • DNA-Binding Proteins
  • DNA-Directed RNA Polymerases
  • Open Reading Frames
  • Transferases

Identity

Scopus Document Identifier

  • 0028269843

PubMed ID

  • 8181716

Additional Document Info

volume

  • 117

issue

  • 2