Localization of a liver-specific enhancer in the apolipoprotein E/C-I/C-II gene locus. Academic Article uri icon

Overview

abstract

  • The sequences necessary for liver-specific expression of the apolipoprotein (apo) E gene have been shown to reside 3' to the gene, within the apoE/C-I/C-II gene cluster, but have not been precisely characterized. Utilizing a transient transfection reporter gene assay based on the apoC-II promoter, we have localized a liver-specific enhancer to its approximate limit dimension of 154 base pairs. This enhancer directed liver-specific expression of an apoE gene construction in transgenic mice. A DNaseI protection assay revealed two footprints over an inverted repeat of a known transcriptionally active motif, TGACCT. DNaseI-sensitive sites were present in three of six repeats of a motif (consensus GCAAACA) which has been postulated to represent the recognition sequence of a hepatic transcriptional activity, HNF-5. This region of DNA may function as a liver-specific enhancer for the entire apoE/C-I/C-II gene cluster.

publication date

  • October 1, 1993

Research

keywords

  • Apolipoproteins C
  • Apolipoproteins E
  • Enhancer Elements, Genetic

Identity

Scopus Document Identifier

  • 0027369115

PubMed ID

  • 8245720

Additional Document Info

volume

  • 34

issue

  • 10