Tetraphenylphosphonium chloride induced MR-visible lipid accumulation in a malignant human breast cell line. Academic Article uri icon

Overview

abstract

  • The effect of the cationic lipophilic phosphonium salt tetraphenylphosphonium chloride (TPP) on a human malignant breast cell line, DU4475, was monitored with proton nuclear magnetic resonance (1H MRS). TPP caused a dose- and time- dependent increase in resonances arising from MR-visible lipid as measured by the CH2/CH3 ratio in the 1-dimensional 1H MR spectrum. Two-dimensional MRS identified increases in the glycerophosphocholine/lysine cross-peak ratio and corresponding decreases in the phosphocholine/lysine ratio in a dose- dependent fashion in TPP-treated cells. Lipid metabolic changes are discussed in the light of other MR experiments, and the data indicate that accumulation of MR-visible lipids may arise from the rearrangement of phospholipids accompanying mitochondrial destruction or from the catabolism of phospholipids associated with early events in the cytotoxic process.

publication date

  • July 3, 1996

Research

keywords

  • Antineoplastic Agents
  • Breast Neoplasms
  • Lipid Metabolism
  • Onium Compounds
  • Organophosphorus Compounds

Identity

Scopus Document Identifier

  • 0030018207

Digital Object Identifier (DOI)

  • 10.1002/(SICI)1097-0215(19960703)67:1<72::AID-IJC13>3.0.CO;2-E

PubMed ID

  • 8690528

Additional Document Info

volume

  • 67

issue

  • 1