Retrovirus-mediated transfer of viral IL-10 gene prolongs murine cardiac allograft survival. Academic Article uri icon

Overview

abstract

  • A murine heterotopic, nonvascularized cardiac allograft model was used to examine the effects of the immunosuppressive cytokine, viral IL-10 (vIL-10), delivered by gene transfer on graft rejection. Retroviral-mediated gene transfer and expression of vIL-10 significantly prolonged allograft survival, without conventional systemic immunosuppression, from 12.1 +/- 0.8 days to 39.4 +/- 2.5 days (p < 0.0001). The effect was specific, dose dependent, and restricted to the site of transplantation. PCR analysis demonstrated specific expression of the transferred gene within the allograft. Analysis of the cellular infiltrate in the allografts showed a reduction in T cells and alloantigen-specific cytotoxic T cells and IL-2 producing helper T cells. Thus, the transient local expression of a gene encoding an immunosuppressive protein within a graft can generate local immunosuppression, making gene therapy a viable approach for facilitating transplantation.

authors

  • Qin, Lihui
  • Chavin, K D
  • Ding, Yaozhong
  • Tahara, Hideaki
  • Favaro, J P
  • Woodward, J E
  • Suzuki, Tadamichi
  • Robbins, P D
  • Lotze, M T
  • Bromberg, J S

publication date

  • March 15, 1996

Research

keywords

  • Gene Transfer Techniques
  • Graft Enhancement, Immunologic
  • Graft Survival
  • Heart Transplantation
  • Interleukin-10
  • Retroviridae
  • Viral Proteins

Identity

Scopus Document Identifier

  • 13344249764

PubMed ID

  • 8690923

Additional Document Info

volume

  • 156

issue

  • 6