Molecular cloning of a gene required for DNA replication in Ustilago maydis. Academic Article uri icon

Overview

abstract

  • TSD2, a gene necessary for DNA synthesis in Ustilago maydis, was cloned by complementation of the temperature sensitive growth defect of a mutant known previously as pol1-1 and renamed here tsd2-1. Linkage analysis established that the cloned fragment contained an allele of tsd2-1 and not a suppressor. DNA sequence determination of the cloned DNA fragment indicated the presence of a single large uninterrupted open reading frame capable of encoding a protein of 845 amino acids without homology to any known gene involved in DNA synthesis. TSD2 was found to be cell cycle-regulated and mRNA levels peaked in early S or G1 phase.

publication date

  • January 27, 1997

Research

keywords

  • Cell Cycle Proteins
  • DNA Replication
  • Fungal Proteins
  • Genes, Fungal
  • Ustilago

Identity

Scopus Document Identifier

  • 0031027479

PubMed ID

  • 9037106

Additional Document Info

volume

  • 253

issue

  • 4