Vascular endothelial growth factor inhibits endothelial cell apoptosis induced by tumor necrosis factor-alpha: balance between growth and death signals. Academic Article uri icon

Overview

abstract

  • A series of experiments was performed to determine whether vascular endothelial growth factor (VEGF), in addition to its endothelial cell specific mitogenic activity, can also protect endothelial cells from toxin-induced programmed cell death. Apoptosis was induced in endothelial cell culture with tumor necrosis factor-alpha (TNF-alpha). Simultaneous exposure of endothelial cells to VEGF resulted in a dose dependent inhibition of apoptosis when evaluated by: (1) direct counting of cells with morphologic features of apoptosis after acridine orange staining; (2) analysis of DNA fragmentation by (a) agarose gel electrophoresis and (b) fluorescence activated cell sorting (FACS); and (3) viability assays dependent upon mitochondrial function. Induction of fibronectin and beta 3 integrin expression in endothelial cells by VEGF suggests that altered adhesion molecule expression may explain this survival effect.

publication date

  • May 1, 1997

Research

keywords

  • Apoptosis
  • Endothelial Growth Factors
  • Endothelium, Vascular
  • Lymphokines
  • Mitogens
  • Tumor Necrosis Factor-alpha

Identity

Scopus Document Identifier

  • 0031149457

PubMed ID

  • 9201618

Additional Document Info

volume

  • 29

issue

  • 5