New natural product families from an environmental DNA (eDNA) gene cluster. Academic Article uri icon

Overview

abstract

  • Uncultured bacteria represent a potentially rich source of new and useful natural products. Studying these natural products requires the development of effective yet straightforward methods to access the small-molecule chemical diversity produced by uncultured bacteria. In this study, DNA extracted directly from soil samples (environmental DNA, eDNA) was used to construct cosmid libraries in Escherichia coli, and these clones were then assayed for the production of antibiosis. A 13 open reading frame (ORF) biosynthetic gene cluster (feeA-M) found in one of the antibacterial active clones, CSLC-2, confers to E. coli the production of two new families of natural products that are derived from long chain N-acyltyrosines. The fee gene cluster and three families of the long chain acyl phenols derived from tyrosine (families 1, 2, and 3) are described.

publication date

  • August 28, 2002

Research

keywords

  • DNA
  • Multigene Family
  • Tyrosine

Identity

Scopus Document Identifier

  • 0037189889

Digital Object Identifier (DOI)

  • 10.1021/ja0268985

PubMed ID

  • 12188643

Additional Document Info

volume

  • 124

issue

  • 34