PML inhibits HIF-1alpha translation and neoangiogenesis through repression of mTOR. Academic Article uri icon

Overview

abstract

  • Loss of the promyelocytic leukaemia (PML) tumour suppressor has been observed in several human cancers. The tumour-suppressive function of PML has been attributed to its ability to induce growth arrest, cellular senescence and apoptosis. Here we identify PML as a critical inhibitor of neoangiogenesis (the formation of new blood vessels) in vivo, in both ischaemic and neoplastic conditions, through the control of protein translation. We demonstrate that in hypoxic conditions PML acts as a negative regulator of the synthesis rate of hypoxia-inducible factor 1alpha (HIF-1alpha) by repressing mammalian target of rapamycin (mTOR). PML physically interacts with mTOR and negatively regulates its association with the small GTPase Rheb by favouring mTOR nuclear accumulation. Notably, Pml-/- cells and tumours display higher sensitivity both in vitro and in vivo to growth inhibition by rapamycin, and lack of PML inversely correlates with phosphorylation of ribosomal protein S6 and tumour angiogenesis in mouse and human tumours. Thus, our findings identify PML as a novel suppressor of mTOR and neoangiogenesis.

publication date

  • August 17, 2006

Research

keywords

  • Hypoxia-Inducible Factor 1, alpha Subunit
  • Neoplasm Proteins
  • Neovascularization, Pathologic
  • Nuclear Proteins
  • Protein Biosynthesis
  • Protein Kinases
  • Repressor Proteins
  • Transcription Factors
  • Tumor Suppressor Proteins

Identity

Scopus Document Identifier

  • 33747488399

PubMed ID

  • 16915281

Additional Document Info

volume

  • 442

issue

  • 7104