Second-end capture in DNA double-strand break repair promoted by Brh2 protein of Ustilago maydis. Academic Article uri icon

Overview

abstract

  • Brh2 plays a central role in the homologous recombination system of Ustilago maydis, mediating delivery of Rad51 to single-stranded DNA. Here we report that Brh2 can pair the displaced strand of a D loop with a complementary single-stranded DNA to form a duplexed, or double, D loop. The reaction emulates the second-end capture step envisioned in models of DNA double-strand break repair. This second-end capture reaction promoted by Brh2 proceeds efficiently when performed in the presence of Rad51 under conditions that block annealing by Rad52, or when the second single-stranded DNA substrate is replaced by double-stranded DNA. In a coupled reaction that requires extension of the D loop more than 200 nt by DNA synthesis in order to reveal a complementary region, Brh2 was also able to promote second-end capture and thus model a synthesis-dependent strand-annealing mechanism.

publication date

  • January 30, 2009

Research

keywords

  • DNA Breaks, Double-Stranded
  • DNA Repair
  • DNA, Fungal
  • Fungal Proteins
  • Recombination, Genetic
  • Ustilago

Identity

PubMed Central ID

  • PMC2663533

Scopus Document Identifier

  • 58649097033

Digital Object Identifier (DOI)

  • 10.1016/j.molcel.2008.12.023

PubMed ID

  • 19187759

Additional Document Info

volume

  • 33

issue

  • 2