Mouse models of osteoarthritis: surgical model of posttraumatic osteoarthritis induced by destabilization of the medial meniscus. Academic Article uri icon

Overview

abstract

  • The surgical model of destabilization of the medial meniscus (DMM) has become a gold standard for studying the onset and progression of posttraumatic osteoarthritis (OA). The DMM model mimics clinical meniscal injury, a known predisposing factor for the development of human OA, and permits the study of structural and biological changes over the course of the disease. In addition, when applied to genetically modified or engineered mouse models, this surgical procedure permits dissection of the relative contribution of a given gene to OA initiation and/or progression. This chapter describes the requirements for the surgical induction of OA in mouse models, and provides guidelines and tools for the subsequent histological, immunohistochemical, and molecular analyses. Methods for the assessment of the contributions of selected genes in genetically modified strains are also provided.

publication date

  • January 1, 2015

Research

keywords

  • Arthritis, Experimental
  • Knee Injuries
  • Menisci, Tibial
  • Osteoarthritis, Knee

Identity

Scopus Document Identifier

  • 84954587576

Digital Object Identifier (DOI)

  • 10.1007/978-1-4939-1619-1_12

PubMed ID

  • 25331049

Additional Document Info

volume

  • 1226