Comparison of transposon and deletion mutants in Mycobacterium tuberculosis: The case of rv1248c, encoding 2-hydroxy-3-oxoadipate synthase. Academic Article uri icon



  • We compared phenotypes of five strains of Mycobacterium tuberculosis (Mtb) differing in their expression of rv1248c and its product, 2-hydroxy-3-oxoadipate synthase (HOAS), with a focus on carbon source-dependent growth rates and attenuation in mice. Surprisingly, an rv1248c transposon mutant on a CDC1551 background grew differently than an rv1248c deletion mutant on the same background. Moreover, the same rv1248c deletion in two different yet genetically similar strain backgrounds (CDC1551 and H37Rv) gave different phenotypes, though each could be complemented. Whole genome re-sequencing did not provide an obvious explanation for these discrepancies. These observations offer a cautionary lesson about the strength of inference from complementation and sequence analysis, and commend consideration of more complex phenomena than usually contemplated in Mtb, such as epigenetic control.

publication date

  • October 19, 2015



  • Aldehyde-Ketone Transferases
  • Bacterial Proteins
  • DNA Transposable Elements
  • Mycobacterium tuberculosis
  • Sequence Deletion
  • Tuberculosis, Pulmonary


PubMed Central ID

  • PMC4667687

Scopus Document Identifier

  • 84948676787

Digital Object Identifier (DOI)

  • 10.1016/

PubMed ID

  • 26547230

Additional Document Info


  • 95


  • 6