The human cytoplasmic dynein interactome reveals novel activators of motility. Academic Article uri icon

Overview

abstract

  • In human cells, cytoplasmic dynein-1 is essential for long-distance transport of many cargos, including organelles, RNAs, proteins, and viruses, towards microtubule minus ends. To understand how a single motor achieves cargo specificity, we identified the human dynein interactome by attaching a promiscuous biotin ligase ('BioID') to seven components of the dynein machinery, including a subunit of the essential cofactor dynactin. This method reported spatial information about the large cytosolic dynein/dynactin complex in living cells. To achieve maximal motile activity and to bind its cargos, human dynein/dynactin requires 'activators', of which only five have been described. We developed methods to identify new activators in our BioID data, and discovered that ninein and ninein-like are a new family of dynein activators. Analysis of the protein interactomes for six activators, including ninein and ninein-like, suggests that each dynein activator has multiple cargos.

publication date

  • July 18, 2017

Research

keywords

  • Carrier Proteins
  • Cell Movement
  • Cytoplasmic Dyneins
  • Dynactin Complex

Identity

PubMed Central ID

  • PMC5533585

Scopus Document Identifier

  • 85026772382

Digital Object Identifier (DOI)

  • 10.7554/eLife.28257

PubMed ID

  • 28718761

Additional Document Info

volume

  • 6