Integration of whole transcriptome spatial profiling with protein markers. Academic Article uri icon

Overview

abstract

  • Spatial transcriptomics and proteomics provide complementary information that independently transformed our understanding of complex biological processes. However, experimental integration of these modalities is limited. To overcome this, we developed Spatial PrOtein and Transcriptome Sequencing (SPOTS) for high-throughput simultaneous spatial transcriptomics and protein profiling. Compared with unimodal measurements, SPOTS substantially improves signal resolution and cell clustering and enhances the discovery power in differential gene expression analysis across tissue regions.

publication date

  • January 2, 2023

Research

keywords

  • Gene Expression Profiling
  • Transcriptome

Identity

Digital Object Identifier (DOI)

  • 10.1038/s41587-022-01536-3

PubMed ID

  • 36593397