Binding of 3H-beta-endorphin to rat brain membranes: characterization of opiate properties and interaction with ACTH. Academic Article uri icon

Overview

abstract

  • The binding of tritiated beta-endorphin (3H-beta-EP) to brain homogenates is described. This has been difficult to achieve due to the lack of availability of 3H-beta-EP and to technical difficulties associated with high non-specific binding of beta-EP. We now report that 3H-beta-EP binding is saturable, stereospecific, has high affinity and is inhibited by sodium. Its dissociation rate is ten-fold longer than that of naloxone. Its regional distribution exhibits interesting differences from naloxone and enkephalin binding. ACTH1-24 appears to displace it more effectively than it displaces 3H-naloxone. The results are discussed in terms of multiple transmitter systems and the multiple opiate receptor hypothesis.

publication date

  • May 30, 1980

Research

keywords

  • Adrenocorticotropic Hormone
  • Brain
  • Endorphins

Identity

Scopus Document Identifier

  • 0018910792

PubMed ID

  • 6256172

Additional Document Info

volume

  • 64

issue

  • 1