LINE-1 retrotransposable element DNA accumulates in HIV-1-infected cells. Academic Article uri icon

Overview

abstract

  • Type 1 long-interspersed nuclear elements (L1s) are autonomous retrotransposable elements that retain the potential for activity in the human genome but are suppressed by host factors. Retrotransposition of L1s into chromosomal DNA can lead to genomic instability, whereas reverse transcription of L1 in the cytosol has the potential to activate innate immune sensors. We hypothesized that HIV-1 infection would compromise cellular control of L1 elements, resulting in the induction of retrotransposition events. Here, we show that HIV-1 infection enhances L1 retrotransposition in Jurkat cells in a Vif- and Vpr-dependent manner. In primary CD4(+) cells, HIV-1 infection results in the accumulation of L1 DNA, at least the majority of which is extrachromosomal. These data expose an unrecognized interaction between HIV-1 and endogenous retrotransposable elements, which may have implications for the innate immune response to HIV-1 infection, as well as for HIV-1-induced genomic instability and cytopathicity.

publication date

  • October 2, 2013

Research

keywords

  • DNA, Viral
  • Endogenous Retroviruses
  • HIV Infections
  • HIV-1
  • Long Interspersed Nucleotide Elements

Identity

PubMed Central ID

  • PMC3838212

Scopus Document Identifier

  • 84888066759

Digital Object Identifier (DOI)

  • 10.1128/JVI.02257-13

PubMed ID

  • 24089548

Additional Document Info

volume

  • 87

issue

  • 24