Protocol for cross-platform characterization of human and murine extracellular vesicles and particles. Academic Article uri icon

Overview

abstract

  • Characterization of isolated extracellular vesicles and particles (EVPs) is crucial for determining functions and biomarker potential. Here, we present a protocol to analyze size, number, morphology, and EVP protein cargo and to validate EVP proteins in both humans and mice. We describe steps for nanoparticle tracking analysis, transmission electron microscopy, single-EVP immunodetection, EVP proteomic mass spectrometry and bioinformatic analysis, and EVP protein validation by ExoELISA and western blot analysis. This allows for EVP cross-validation across different platforms. For complete details on the use and execution of this protocol, please refer to Hoshino et al.1.

publication date

  • December 13, 2023

Research

keywords

  • Extracellular Vesicles
  • Proteomics

Identity

Digital Object Identifier (DOI)

  • 10.1016/j.xpro.2023.102754

PubMed ID

  • 38096060

Additional Document Info

volume

  • 5

issue

  • 1